Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Highlights
Quantify as little 20 femtograms of human DNA in as little as 1 µl of sample.
High specificity and sensitivity for human DNA in a background of non-target DNA.
Fast and simple: add samples to the PreMix... and quantify.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
Quantify as little 20 femtograms of human DNA in as little as 1 µl of sample.
High specificity and sensitivity for human DNA in a background of non-target DNA.
Fast and simple: add samples to the PreMix... and quantify.
Product Description
The Femto Human DNA Quantification Kit can detect and quantify human DNA with high specificity and sensitivity. Human DNA can be reliably quantified in a background of non-human DNA, such as fungal, bacterial, animal, plant DNA, etc. This is essential for downstream applications that require accurate DNA input amounts such as STR analysis. The Femto Human DNA Quantification Kit dependably quantifies as little as 20 fg from 1 µL of DNA purified from biological liquids, human cell cultures, anthropological samples, or forensic samples.
Technical Specifications
Detection Range
20 fg to 20 ng DNA
Equipment
Real-time quantitative PCR system, vortex mixer, microcentrifuge, PCR tube strip or PCR plate; optically transparent sealing film for PCR plate or tube strip caps
Since this kit is so sensitive, avoiding possible sources of contamination is key for a successful qPCR. - Work in a clean environment and decontaminate the benchtop and pipettes with a 10% bleach solution. - Wipe down the areas with ethanol to remove the trace amounts of bleach and allow the area to try. - Use filtered tips. - Take care to ensure that pipetting is efficient and accurate. Calibrate your pipettes regularly. - Add standards directly into the premix and pipet up and down to flush out any remaining liquid from the tip. - Avoid pipetting onto the walls as this may skew the results. - The SYTO9 dye present within the premix is sensitive to light, so working quickly and diligently is recommended.
