Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Highlights
Quantify as little 20 femtograms of bacterial DNA in as little as 1 µl of sample.
High specificity and sensitivity for bacterial DNA in a background of non-target DNA.
Fast and simple: add samples to the PreMix... and quantify.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
Quantify as little 20 femtograms of bacterial DNA in as little as 1 µl of sample.
High specificity and sensitivity for bacterial DNA in a background of non-target DNA.
Fast and simple: add samples to the PreMix... and quantify.
roduct Description
The Femto Bacterial DNA Quantification Kit can be used to detect and quantify bacterial DNA with high specificity and sensitivity. Bacterial DNA can be reliably quantified in a background of non-bacterial DNA such as fungal, animal, and plant DNA, etc. This is essential for downstream applications that require accurate DNA input including quantifying bacteria DNA template for Next-generation sequencing library preparation and metagenomic analysis amongst others. With the Femto Bacterial DNA Quantification Kit, one can dependably quantify as little as 20 fg DNA from 1 µl purified from biological liquids, bacterial cultures, or environmental DNA samples.
Technical Specifications
Detection Range
20 fg to 20 ng DNA
Equipment
Real-time quantitative PCR system,vortex, microcentrifuge, PCR tube strip or PCR plate, optically transparent sealing film for PCR plate or tube strip caps
Since this kit is so sensitive, avoiding possible sources of contamination is key for a successful qPCR. - Work in a clean environment and decontaminate the benchtop and pipettes with a 10% bleach solution. - Wipe down the areas with ethanol to remove the trace amounts of bleach and allow the area to try. - Use filtered tips. - Take care to ensure that pipetting is efficient and accurate. Calibrate your pipettes regularly. - Add standards directly into the premix and pipet up and down to flush out any remaining liquid from the tip. - Avoid pipetting onto the walls as this may skew the results. - The SYTO9 dye present within the premix is sensitive to light, so working quickly and diligently is recommended.
E. coli strain JM109 is a derivative of strain K12 and is also called K12 JM109. The genome size of the original genome of H12 is 4.64 Mb (ref). Ref: https://www.ncbi.nlm.nih.gov/pubmed/9278503
The standards included in the Femto Bacterial DNA Quantification kit are serial dilutions of E. coli genomic DNA. Each E. coli genome has about 7 copies of 16S rRNA gene. One can back calculate from the amount of genomic DNA to determine genomic copies, and then multiply by a factor of 7 to determine total copies of the 16S rRNA gene.
Genome copy # = DNA (g) / (g_to_bp const. x genome size) With DNA (g) = 20 ng g to bp const = 1.096 x 10^-21 g genome size = 4.6 x 10^6 bp 20 x 10^-9 g / (1.096 x 10^-21 g/bp x 4.6 x 10^6 bp) = 4.0 x 10^6 copies
