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Quick-DNA/RNA Viral 96 Kit
Highlights
Quick recovery of viral DNA/RNA from plasma, serum and other samples.
Omits the use of organic denaturants and proteases.
High-quality viral DNA/RNA is ready for RT-PCR, sequencing, etc.
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

  • Quick recovery of viral DNA/RNA from plasma, serum and other samples.
  • Omits the use of organic denaturants and proteases.
  • High-quality viral DNA/RNA is ready for RT-PCR, sequencing, etc.
 

Description

The Quick-DNA/RNA Viral 96 Kit is a fast viral DNA/RNA purification kit that provides rapid, large scale isolation of high-quality viral DNA and/or RNA from a wide range of biological sources. It can be used to successfully isolate viral DNA and/or RNA from cell-free body fluids, as well as cellular suspensions at concentrations ≤1x105 cells/ml. The kit has been rigorously tested and used to isolate viral RNA from samples containing enteroviruses, rhinoviruses, coronaviruses, HIV, HCV, influenza A virus, flaviviruses, measles virus, parainfluenza virus and parvovirus (a ssDNA virus). The Quick-DNA/RNA Viral 96 Kit employs a single buffer system that facilitates viral particle lysis and allows for the subsequent DNA/RNA adsorption onto the matrix of the Zymo-Spin I-96 Plate. The DNA/RNA is washed, then eluted with DNase/RNase-Free Water. The eluted DNA/RNA is suitable for use in various subsequent procedures including RT-PCR.

Performance

Technical Specifications
EquipmentCentrifuge with microplate carriers with height tolerance of 60 mm (2.36 inches)
PurityHigh-quality nucleic acids are ready for Next-Gen sequencing, RT/qPCR, hybridization, etc.
Sample SourcePlasma, serum, saliva, urine, blood, cell culture media, cellular suspensions, biopsies, swab, and fecal samples
Size Range50 nt to ~200 kb
Yield5 µg DNA and 10 µg RNA

Resources

Protocol: 
Datasheet: 
SDS (MSDS): D7022 | D7023

FAQ

The purpose of beta-mercaptoethanol is to help with deproteination. It is not necessary if you are working with simple samples such as swabs. It is recommended if you are working with protein rich samples such as plasma, serum, blood, saliva, sputum, etc.

Yes, these samples are compatible.

Yes, this kit will co-purify some DNA.

Most downstream application methods for viral detection do not require DNase treatment during purification. If necessary, DNase treatment can be perform post-purification and additional components can be purchased separately (i.e., DNase I, DNA Digestion Buffer, RNA Prep Buffer and RNA Wash Buffer).

Yes, the change from clear to yellow is a result of oxidation and will not affect the performance of the buffer.

The Viral RNA Buffer is guaranteed for one year from the date of purchase even with the addition of beta-mercaptoethanol. Just be sure to close the bottle cap tightly to prevent evaporation.

In most cases, it could be because the samples contain high amounts of protein or cellular debris. To help prevent this in future preps, it’s best to add beta-mercaptoethanol to the Viral RNA buffer, and/or implement a Proteinase K digestion step.

For optimal results and detection of viral target, collect sample in DNA/RNA Shield and perform Proteinase K treatment. In addition, add beta-mercaptoethanol to the Viral RNA Buffer prior to purification, as well as perform all steps at room temperature and centrifugation speeds at 10,000-16,000 x g to ensure no buffer retention.

This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.

주문정보

CAT.No 품명 규격 비고
D7022 Quick-DNA/RNA Viral 96 Kit 2 x 96 Preps
D7023 Quick-DNA/RNA Viral 96 Kit 4 x 96 Preps