Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Highlights
Depletes Host and Enriches for Bacteria: Increase the ratio of bacteria to host DNA.
Fast: Only 30 minutes of hands-on time for purification of high-quality DNA.
Accurate: Unbiased cellular lysis for accurate analysis of the microbial profile.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
Depletes Host and Enriches for Bacteria: Increase the ratio of bacteria to host DNA.
Fast: Only 30 minutes of hands-on time for purification of high-quality DNA.
Accurate: Unbiased cellular lysis for accurate analysis of the microbial profile.
Product Description
The HostZERO Microbial DNA Kit is designed to overcome the challenge of contaminating host nucleic acids in microbial samples. This kit uses a novel method to reduce the amount of contaminating host DNA by selectively lysing the eukaryotic cells and degrading this DNA prior to total DNA purification. Paired with Zymo Research’s non-biased purification technology, the HostZERO Microbial DNA Kit allows for the exclusive capture of DNA from living microbial cells in a biological sample. This new technology is able to reduce the presence of human DNA in a saliva sample from 65% (untreated sample) to less than 1% (treated sample). Concurrently, the depletion process utilizes the ZymoBIOMICS non-biased DNA isolation technology for accurate data representation. Finally, the HostZERO Microbial DNA Kit recovers the highest amount of bacterial DNA as compared to other methods. By removing the presence of host DNA and reducing bias in purification, the HostZERO Microbial DNA Kit produces the highest-quality data for microbial samples.
Technical Specifications
DNA Purity
High-quality DNA is eluted with ZymoBIOMICS® DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation Sequencing.
DNA Recovery
Greater than 85% of total bacterial DNA is effectively recovered with greater than 90% of eukaryotic host DNA depleted.
Processing Time
Bacterial DNA is isolated from a single sample in less than 90 minutes.
Required Equipment
Microcentrifuge, vortex, tube rotator, and high-speed cell disruptor (recommended).
Sample Input
Up to 200 µl liquid sample.
Sample Type
Saliva, swabs, and bodily fluids from eukaryotic hosts with intact (living) bacteria cells.
Studies where shotgun metagenomic sequencing will be used to produce the microbial profile of a sample high in host DNA. To reduce the number of reads that will go to the host DNA, host DNA must first be depleted.
Feces have not been validated. Stool from a healthy donor is not expected to contain a high abundance of host DNA. However, if the stool is from a donor with a bowel related illness (e.g. ulcerative colitis), then host depletion may be useful.
Samples such as saliva, swabs, and bodily fluids from eukaryotic hosts with intact bacteria cells are directly compatible with this kit. Pre-treatment is required for whole blood and solid tissues. Samples in DNA/RNA Shield are not compatible.
No, the HostZero kit cannot be used to deplete host DNA from viral samples. Viral DNA will be removed with host DNA during the depletion step. The kit can only be used for host depletion from microbial samples.
