Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Product Description
Micrococcal Nuclease cleaves single-stranded and double-stranded DNA and RNA. Complete digestion with Micrococcal Nuclease yields mono- and oligonucleotides with 3′-phosphates.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Product Description
Micrococcal Nuclease cleaves single-stranded and double-stranded DNA and RNA. Complete digestion with Micrococcal Nuclease yields mono- and oligonucleotides with 3′-phosphates.
Technical Specifications
Concentration
0.1 U/µl
Enzyme Commission Number
(E.C. 3.1.31.1)
Enzyme Inactivation
EDTA or EGTA in molar excess of CaCl2.
Storage
Store at -20°C for up to 12 months. Avoid repeated freeze/thawing. Prolonged storage should be ≤ -70 °C.
Unit Definition
One unit (U) will produce 1.0 µmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37°C, based on EM/260 = 10,000 for the mixed nucleotides.
• Atlantis dsDNase is a double-stranded DNA specific endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. Increasing the incubation time with Atlantis dsDNAse will increase efficiency and generate more mono-nucleosomal DNA. • Micrococcal Nuclease (MNase) is a single and double-stranded DNA and RNA endonuclease. MNase is more specific for single-stranded nucleic acids, but cleavage is biased towards AT-rich and AU-rich sequences. MNase will result in more robust digestion compared to Atlantis dsDNase. Increasing the incubation time with MNase will increase efficiency of this enzyme and generate more mono-nucleosomal DNA. • The enzyme selection depends on desired downstream applications.
Once the nuclei are isolated, the enzyme sensitivity should be similar between different cell types. However, some cells lines will be more sensitive to the detergent in the Nuclei Prep Buffer and thus result in a loss of nuclei. You can dilute the Nuclei Prep Buffer 1:1 with MN Digestion Buffer prior to nuclei isolation.
MNase is an endo-exonuclease that preferentially cleaves at AT or AU-rich regions, yielding mono-nucleotides and oligonucleotides with terminal 3'-phosphates.
