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DNase I Set (RNase-free)
Highlights
Highest Activity: Complete and efficient DNA digestion for sensitive downstream applications like RNA sequencing.
RNase-free and Protease-free: Rigorously tested and guaranteed to be free of contaminating RNases and proteases.
Exceptional Stability: Maintains activity after vigorous vortexing and multiple freeze-thaw cycles.
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

  • Highest Activity: Complete and efficient DNA digestion for sensitive downstream applications like RNA sequencing.
  • RNase-free and Protease-free: Rigorously tested and guaranteed to be free of contaminating RNases and proteases.
  • Exceptional Stability: Maintains activity after vigorous vortexing and multiple freeze-thaw cycles.

Product Description

Zymo Research DNase I is a recombinant endonuclease that cleaves single- and double-stranded DNA. It digests DNA more efficiently than other DNase I, making it optimal for sensitive applications like NGS (RNA-seq) and PCR. It is extensively purified, and quality controlled to ensure RNase-free and protease-free.

Zymo Research DNase I is lyophilized and supplied with DNA digestion buffer. It dissolves in water within seconds and remains stable for up to 10 freeze-thaw cycles. Customized, OEM, and bulk orders are available.

Technical Specifications

Unit DefinitionOne Kunitz causes an increase in absorbance at 260 nm of 0.001 per minute per ml, at 25°C, pH 7.5, when acting on salmon sperm DNA according to the assay method of Kunitz. One Unit of DNase I is equivalent to 12 Kunitz.
StorageAfter reconstitution, place on ice until ready to use or store frozen aliquots (-20°C).
ReconstitutionAdd DNase/RNase-free water to the lyophilized DNase I, mix by gentle inversion. Avoid phosphate buffer and calcium chelators.
InactivationHeat inactivate at 75 °C for 10 min with 5 mM EDTA.

Resources

  • Remove contaminating DNA from RNA or protein samples
  • Clean up of RNA samples prior to RT-PCR
  • DNA-free RNA sample preparation prior to RNA-seq
  • Remove DNA templates after in vitro transcription
  • DNA labeling by nick-translation
  • Reduce viscosity in cell culture or cell lysate
  • DNase I footprinting

주문정보

CAT.No 품명 규격 비고
E1010 DNase I Set 250 U
E1011 DNase I Set 1500 U
E1012 DNase I Set 5 x 1500 U